While there was no shortage of memorable moments at the 2016 Golden Globes, hawk-eyed viewers were particularly curious about Leonardo DiCaprio's reaction to Lady Gaga's award win. As the singer passed his table to accept the trophy for best actress in a miniseries, there seemed to be a very awkward encounter.

Leonardo DiCaprio appeared startled by Lady Gaga. (via GIPHY)

In a short clip that quickly went viral, Lady Gaga walked toward the stage to collect her award and brushed past a laughing Leonardo – who was caught pulling what seemed to be a startled expression at seeing the "Just Dance" hitmaker as she continued to make her way through the crowd.

However, the 41-year-old has now revealed what really happened during the humorous moment caught on-camera, and admitted he didn't realize Lady Gaga was walking past him.

"Oh lord – that's trending huh," he laughed when shown the video by Entertainment Tonight. "I just didn't know what was passing me, that's all!"

It proved to be a particularly exciting evening for the Titanic actor, who picked up the trophy for Best Actor following his great performance in The Revenant.

Leonardo, who plays real-life frontiersman Hugh Glass in the blockbuster, beat out fellow nominees Eddie Redmayne, Michael Fassbender, Will Smith and Bryan Cranston for the trophy.

TAP IMAGE TO ENLARGE:Leonardo also snagged a trophy at the 2016 Golden Globes.

Taking to the stage, he received a standing ovation from the room, who cheered his achievement.

"What an incredible honour, wow, that meant a lot," he said. "Thank you so much, to the other fantastic performances in this category. What a terrific year in film."

To director Alejandro González Iñárritu, he added, "Thank you for allowing me to be a part of this journey with you. Your leadership, your vision, your acute attention to making every day so visceral and real for us – I've never had an experience like this in my entire life."

The actor then described co-star Tom Hardy as "a beast and unbelievable talent" before thanking his loved ones. "My parents, I love you dearly," said Leonardo. "My friends, you know who you are."